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Accueil > Français > Annuaire > Permanents > BLOT Nicolas

Nicolas Blot’s page in english

par FredDelbac - publié le , mis à jour le




Université Clermont Auvergne, Clermont-Ferrand, France

  • Researcher in Parasitology
  • Lecturer (Maître de Conférences) in Microbiology
  • Scientific committee of the Maison Pour la Science en Auvergne
  • Master’s Degree in Engineering (Biochemistry), MSc in Genetics
  • PhD in Microbiology
  • HDR (post-doctoral degree)

Laboratoire Microorganismes : Génome et Environnement

UMR6023 CNRS-UCA

Campus Universitaire des Cézeaux

1 Impasse Amélie Murat

TSA 60026

CS 60026

63178 AUBIERE CEDEX

France

Phone : +33 473 40 74 57 (currently offline)

Fax : +33 473 40 76 70

E-mail : nicolas.blot@uca.fr

  • Office 1117 (1st floor)

Currently invited scientist by the CIRAD

UMR Peuplements Végétaux et Bioagresseurs en Milieu Tropical

CIRAD - 3P

7 chemin de l’IRAT

Ligne Paradis

97410 SAINT-PIERRE

France (Réunion Island)

Phone, from France : 02 62 49 92 06
from abroad : +262 262 49 92 06

E-mail : nicolas.blot@uca.fr

  • Office : 0-12



RESEARCH PROJECT

Research area : Host-Parasite Interactions

- HONEYBEE HEALTH : The microsporidian parasites of Apis mellifera

  • Interactions Nosema ceranae-pesticides
  • Adaptations and pathogenicity of the microsporidia
  • Diversity of the microsporidian parasites

- MEDIA TALKED ABOUT US :


- OUR PARTNERS :



PREVIOUS RESEARCH PROJECTS


- HUMAN INTESTINAL PARASITISM : genome and mitochondrial-like organelle of the human anaerobic parasite Blastocystis

  • with the LMGE (Clermont-Fd)


- OCEANIC PLANKTON : fonctional diversity, physiology and stress response of marine cyanobacteria



- CHROMATIN STRUCTURE AND PHYSIOLOGY : regulation of the transcription by the DNA superhelical density and by chromatin proteins in bacteria



- BACTERIAL PHYTOPATHOGENICITY : virulence, regulation and transport of the plant pectin degradation products in the ’soft-rot’ agent Dickeya dadantii



PUBLICATIONS

  • Faure D, Bonin P, Duran R and the Microbial Ecology EC2CO consortium (2015) Environmental microbiology as a mosaic of explored ecosystems and issues. Environ Sci Pollut Res. 22 :13577-13598
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  • Aufauvre J, Misme-Aucouturier B, Viguès B, Texier C, Delbac F and Blot N (2014) Transcriptome analysis of the honeybee response to Nosema ceranae and insecticides. PLoS ONE. 9:e91686
  • Roudel M, Aufauvre J, Corbara B, Delbac F and Blot N (2013) New insights on the genetic diversity of the honeybee parasite Nosema ceranae based on multilocus sequence analysis. Parasitology. 140:1346-1356
  • Medrzycki P, Giffard H, Aupinel P, Belzunces LP, Chauzat MP, Claßen C, Colin ME, Dupont T, Girolami V, Johnson R, Le Conte Y, Lückmann J, Marzaro M, Pistorius J, Porrini C, Schur A, Sgolastra F, Delso NS, van der Steen JJM, Wallner K, Alaux C, Biron DG, Blot N, Bogo G, Brunet JL, Delbac F, Diogon M, El Alaoui H, Provost B, Tosi S and Vidau C (2013) Standard methods for toxicology research in Apis mellifera. Journal of Apicultural Research. 52(4)
  • Shukla A, Biswas A, Blot N, Partensky F, Karty JA, Hammad LA, Garczarek L, Gutu A, Schluchter WM, and Kehoe DM (2012) Phycoerythrin-specific bilin lyase-isomerase controls blue-green chromatic acclimation in marine Synechococcus. Proc Natl Acad Sci USA. 109:20136-20141
  • Mella-Flores D, Six C, Ratin M, Partensky F, Boutte C, Le Corguillé G, Marie D, Blot N, Gourvil P, Kolowrat C and Garczarek L (2012) Prochlorococcus and Synechococcus have evolved different adaptive mechanisms to cope with light and UV stress. Front Microbiol. 3:285
  • Aufauvre J, Biron DG, Vidau C, Fontbonne R, Roudel M, Diogon M, Viguès B, Belzunces LP, Delbac F and Blot N (2012) Parasite-insecticide interactions : a case study of Nosema ceranae and fipronil synergy on honeybee. Scientific Reports. 2:326.
  • Blot N, Mella-Flores D, Six C, Le Corguillé G, Boutte C, Peyrat A, Monnier A, Ratin M, Gourvil P, Campbell D A and Garczarek L (2011) Light history influences the response of the marine cyanobacterium Synechococcus sp. WH7803 to oxidative stress. Plant Physiol. 156:1934-1954
  • Vidau C, Diogon M, Aufauvre J, Fontbonne R, Viguès B, Brunet J-L, Texier C, Biron DG, Blot N, El Alaoui H, Belzunces LP and Delbac L (2011) Exposure to sublethal doses of Fipronil and Thiacloprid highly increases mortality of honeybees previously infected by Nosema ceranae. PLoS ONE. 6:e21550.
  • Denoeud F, Roussel M, Noel B, Wawrzyniak I, Da Silva C, Diogon M, Viscogliosi E, Brochier-Armanet C, Couloux A, Poulain J, Segurans B, Anthouard V, Texier C, Blot N, Poirier P, Choo NG, Tan KS, Artiguenave F, Jaillon O, Aury JM, Delbac F, Wincker P, Vivares CP and El Alaoui H. (2011) Genome sequence of the stramenopile Blastocystis, a human anaerobic parasite. Genome Biol. 12:R29.
  • Polonais V, Mazet M, Wawrzyniak I, Texier C, Blot N, El Alaoui H, and Delbac F (2010) The human microsporidian Encephalitozoon hellem synthesizes two spore wall polymorphic proteins useful for epidemiological studies. Infect. Immun. 78:2221-2230.
  • Kolowrat C, Partensky F, Mella-Flores D, Le Corguillé G, Boutte C, Blot N, Ratin M, Ferréol M, Lecomte X, Gourvil P, Lennon JF, Kehoe DM, and Garczarek L (2010) . Ultraviolet stress delays chromosome replication in light/dark synchronized cells of the marine cyanobacterium Prochlorococcus marinus PCC9511. BMC Microbiol. 10:204.
  • Blot N, Wu XJ, Thomas J-C, Zhang J, Garczarek L, Böhm S, Tu JM, Zhou M, Plöscher M, Eichacker L, Partensky F, Scheer H, and Zhao KH (2009) Phycourobilin in trichromatic phycocyanin from oceanic cyanobacteria is formed post-translationally by a phycoerythrobilin lyase-isomerase. J. Biol. Chem. 284:9290-9298.
  • Garczarek L, Dufresne A, Blot N, Cockshutt AM, Peyrat A, Campbell DA, Joubin L, and Six C (2008) Function and evolution of the psbA gene family in marine Synechococcus : Synechococcus sp. WH7803 as a case study. ISME J. 2:937-953.
  • Six C, Thomas J-C, Garczarek L, Ostrowski M, Dufresne A, Blot N, Scanlan DJ, and Partensky F (2007) Diversity and evolution of phycobilisomes in marine Synechococcus spp. - a comparative genomics study. Genome Biol. 8:R259.
  • Lautier T, Blot N, Muskhelishvili G, and Nasser W (2007) Integration of two essential virulence modulating signals at the Erwinia chrysanthemi pel gene promoters : a role for Fis in the growth-phase regulation. Mol. Microbiol. 66:1491-1505.
  • Lang B, Blot N, Bouffartigues E, Buckle M, Geertz M, Gualerzi CO, Mavathur R, Muskhelishvili G, Pon, CL, Rimsky S, Stella S, Babu MM, and Travers A (2007) High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes. Nucleic Acids Res. 35:6330-6337.
  • Blot N, Mavathur R, Geertz M, Travers A, and Muskhelishvili G (2006) Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome. EMBO Rep. 7:710-715.
  • Rochman M, Blot N, Dyachenko M, Glaser G, Travers A, and Muskhelishvili G (2004) Buffering of stable RNA promoter activity against DNA relaxation requires a far upstream sequence. Mol. Microbiol. 53:143-152.
  • Pellinen T, Ahlfors H, Blot N, and Condemine G (2003) Topology of the Erwinia chrysanthemi oligogalacturonate porin KdgM. Biochem. J. 372:329-334.
  • Blot N, Berrier C, Ghazi A Hugouvieux-Cotte-Pattat N, and Condemine G (2002) Characterization of KdgM, a porin of a new family, in the phytopathogenic bacteria Erwinia chrysanhtemi. J. Biol. Chem. 277:7936-7944.
  • Hugouvieux-Cotte-Pattat N, Blot N, and Reverchon S (2001) Identification of TogMNAB, an ABC transporter which mediates the uptake of pectic oligomers in Erwina chrysanthemi 3937. Mol. Microbiol. 41:1113-1123.

- Scientific popularization papers

  • Roudel M, and Blot N (2012) Etude du polymorphisme génétique du parasite microsporidien Nosema ceranae. Auvergne Sciences, Le Magazine. Août.
  • Vidau C, Diogon M, Aufauvre J, Fontbonne R, Viguès B, Brunet J-L, Texier C, Biron DG, Blot N, El Alaoui H, Belzunces LP, and Delbac L (2011) Déclin des colonies d’abeilles et origine multifactorielle : cas de Nosema ceranae et des insecticides. La Santé de l’Abeille. 245:417-426.
  • El Alaoui H, Poincloux D, Diogon M, Roussel M, Texier C, Wawrzyniak I, Blot N, Vivarès CP and Delbac F(2009) Des insectes à l’homme : la formidable réussite infectieuse des microsporidies. La Santé de l’Abeille. 232:285-293

- Speeches and posters

click here




TEACHING AND SUPERVISION

- TEACHING AT THE UNIVERSITE CLERMONT AUVERGNE

  • Bachelors Degree in Life Sciences
  • Masters Degree in Microbiology
    • Lectures and practical courses in microbiology, bacteriology, microbial physiology, microbial diseases and pathogens, virology, molecular biology, quality control…


- MAISON POUR LA SCIENCE EN AUVERGNE

  • Organization of scientific trainings for teachers in primary and secondary schools
  • Trainer in various programs :
    • pollination and honeybee
    • light in science : pigments and photosynthesis
    • growth and development
    • aquatique ecosystems and water quality
    • phylogeny ...

-  SUPERVISION OF PhD STUDENTS

  • Regis ROUZE : Interactions between pathogenes and pesticide mixtures in the honeybee, 2017-2019, 2014-2017
  • Julie AUFAUVRE : Impact of pesticides on Nosema ceranae virulence, defended dec. 19th, 2013
  • Mathieu ROUDEL : Diversity of the Nosema parasites of the honeybee, defended dec. 12th, 2013
  • Michael ROUSSEL : Genome sequencing of the anaerobic gut parasite Blastocystis sp. (ST7) : towards a better knowledge of the metabolism of the mitochondria-like-organelle, defended sept. 27th, 2011 (co-supervision

- SUPERVISION OF OTHER STUDENTS

  • Undergraduate, Postgraduate, Bachelor and Master students…

- SCIENTIFIC ANIMATION

  • For general public (scientific events, media...)
  • For young public (schools, youth organizations...)
  • For student (scientific events, forums...)






    • Keywords : Scientific Research Laboratory - Microbiology, parasitology, molecular biology, genomics, transcriptomics, environment, contaminants and contamination, diversity, stress, adaptation - Hive and honeybee diseases, bee and hymenopteran health, pollinators decline, mortality, pathogens, parasite, microsporidia, Nosema and nosemosis, Varroa, pesticides, neurotoxic insecticides, neonicotinoids, glyphosate, synergy and cocktail effect - DNA, chromatin, regulation, of transcription and virulence, see and ocean, photosynthesis, phytoplancton, cyanobacteria, biodiversity hot-spot, population diversity, genotyping